Microtomy

Leica 3050 Cryostats

The Leica CM3050 cryostat is used to cut sections of frozen samples embedded in a tissue freezing medium. The system located in DBI is equipped with the CryoJane® Tape-Transfer System for the collection of flat, wrinkle free sections as thin as 2µm that are bonded to an adhesive slide.

Locations: APB 141B and Wolf Hall 267

Ultramicrotomes

The Leica UC7 and Reichert-Jung Ultracut E are used to cut ultrathin and semithin sections of resin-embedded samples for electron microscopy.

Location: APB 141H

Vibratome

The Micro-Cut H1200 vibrating microtome enables sectioning of fresh or fixed tissue without the need for typical specimen processing and embedding or freezing.

Location: APB 141B

Specifications:

  • Section thickness range of 10 to 1000 microns
  • Specimen diameter up to 40mm
  • Blade has a forward travel up to 40mm but can be limited to 35, 30 or 25mm for smaller specimens
  • Automatic blade return after each cutting cycle

Rotary Microtomes

The Microm HM 335E is a power assisted, rotary microtome for sectioning of paraffin embedded specimens.

Location: APB 141B